Sundar Lab

These seed lines are currently available from the ABRC

Please see our article in the journal Development

Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis
Gabriela C. Pagnussat 1,*, Hee-Ju Yu 1,3,*, Quy A. Ngo 1, Rajani Sarojam 1, Sevugan Mayalagu 1,‡, Cameron S. Johnson 1, Arnaud Capron 1, Li-Fen Xie 4∞, De Ye 4§, Venkatesan Sundaresan 1,2,†

Identity of genes disrupted by Ds::Kan^R in female gametophyte mutants
grouped by phenotypic category with the transmission ratios for each mutant gene

General Defect Observed	Mutant Line ID	DS Element Location	Flanking Sequence	Kan^R: Kan^S Crossed as Female	Phenotype Observed

In nuclear division phase of megagametogenesis	EDA1	At1g59680	Seq	0.0092	Arrested at 2-nuclear stage
	EDA 2	At2g18080*	Seq	0.0000	Arrested at 2-nuclear stage
	EDA 3	At2g34860	Seq	0.0029	Arrested at 2-nuclear stage
	EDA 4	At2g48140	Seq	0.0000	Arrested at 4 nuclear stage
	EDA 5	At3g03650	Seq	0.1900	Arrested at 4 nuclear stage
	EDA 6	At3g23440	Seq	0.3500	Arrested at 2-nuclear stage
	EDA 7	At3g56990	Seq	0.0090	Arrested at 4 nuclear stage
	EDA 8	At4g00310*	Seq	0.3300	Arrested at stage FG1
	EDA 9	At4g34200	Seq	0.0000	Arrested at 2-nuclear stage

Abnormal nuclear numbers and positions	EDA 10	At1g01960	Seq	0.4700	Variant nuclear number or no embryo sac
	EDA 11	At1g55420	Seq	0.1700	Variant nuclear number and positions
	EDA 12	At2g35950	Seq	0.5200	Variant nuclear number and positions
	EDA 13	At2g47990	Seq	0.0000	Variant nuclear number and positions
	EDA 14	At3g60360	Seq	0.0000	Abnormal positions and size 4 nuclear stage
	EDA 15	At4g14790	Seq	0.4400	Variant nuclear number and positions

Arrested at varying stages of embryo sac development	EDA 16	At1g61140	Seq	0.4600	Aberrant embryo sacs and with un-fused polar nuclei
	EDA 17	At1g72970	Seq	0.1800	Aberrant embryo sac and arrested at 2-nuclear stage
	EDA 18	At2g34920	Seq	0.3500	Aberrant embryo sacs and with un-fused polar nuclei
	EDA 19	At2g47990	Seq	0.0380	Aberrant embryo sacs and with un-fused polar nuclei
	EDA 20	At4g00020	Seq	0.2300	Aberrant embryo sacs and unfertilized ovules
	EDA 21	At4g13235	Seq	0.4900	Aberrant embryo sacs and with un-fused polar nuclei
	EDA 22	At5g05920	Seq	0.3000	Arrested at 4-nuclear stage and un-fused polar nuclei
	EDA 23	At5g44700	Seq	0.1974	Arrested at 2 nuclear stage and un-fused polar nuclei

Unfused polar nuclei	EDA 24	At1g70540	Seq	0.0208	Un-fused polar nuclei
	EDA 25	At1g72440	Seq	0.5846	Un-fused polar nuclei
	EDA 26	At2g01730	Seq	0.4100	Un-fused polar nuclei
	EDA 27	At2g20490	Seq	0.0000	Un-fused polar nuclei
	EDA 28	At2g34790	Seq	0.4100	Un-fused polar nuclei
	EDA 29	At2g35940	Seq	0.5100	Un-fused polar nuclei
	EDA 30	At3g03810	Seq	0.1200	Un-fused polar nuclei
	EDA 31	At3g10000	Seq	0.4600	Un-fused polar nuclei
	EDA 32	At3g62210	Seq	0.4773	Un-fused polar nuclei
	EDA 33	At4g00120	Seq	0.5200	Un-fused polar nuclei
	EDA 34	At4g00140	Seq	0.1573	Un-fused polar nuclei
	EDA 35	At4g05440	Seq	0.0350	Un-fused polar nuclei
	EDA 36	At4g13890	Seq	0.2500	Un-fused polar nuclei
	EDA 37	At4g13890	Seq	0.2500	Un-fused polar nuclei
	EDA 38	At4g14040	Seq	0.6840	Un-fused polar nuclei
	EDA 39	At4g33050	Seq	0.6200	Un-fused polar nuclei
	EDA 40	At4g37890	Seq	0.5844	Un-fused polar nuclei
	EDA 41	At5g52460	Seq	0.2900	Un-fused polar nuclei

Defects in fertilization	UNE 1	At1g29300	Seq	0.2000	Defects in pollen tube attraction
	UNE 2	At1g78130	Seq	0.2900	Unfertilized ovules but normal pollen tube attraction
	UNE 3	At2g01110	Seq	0.7143	Unfertilized ovules but normal pollen tube attraction
	UNE 4	At2g12940	Seq	0.3500	Defects in pollen tube attraction
	UNE 5	At2g47470	Seq	0.7813	Unfertilized ovules but normal pollen tube attraction
	UNE 6	At3g03340	Seq	0.8200	Unfertilized ovules but normal pollen tube attraction
	UNE 7	At3g03690	Seq	0.2800	Unfertilized ovules but normal pollen tube attraction
	UNE 8	At3g05690	Seq	0.1800	Unfertilized ovules but normal pollen tube attraction
	UNE 9	At3g10560	Seq	0.3400	Defects in pollen tube attraction
	UNE 10	At4g00050	Seq	0.4500	Unfertilized ovules but normal pollen tube attraction
	UNE 11	At4g00080	Seq	0.6417	Defects in pollen tube attraction
	UNE 12	At4g02590	Seq	0.5200	Unfertilized ovules but normal pollen tube attraction
	UNE 13	At4g12620	Seq	0.3700	Unfertilized ovules but normal pollen tube attraction
	UNE 14	At4g12860	Seq	0.5800	Defects in pollen tube attraction
	UNE 15	At4g13560	Seq	0.4900	Defects in pollen tube attraction
	UNE 16	At4g13640	Seq	0.2400	Unfertilized ovules but normal pollen tube attraction
	UNE 17	At4g26330	Seq	0.2300	Unfertilized ovules but normal pollen tube attraction
	UNE 18	At5g02100	Seq	0.1400	Unfertilized ovules but normal pollen tube attraction

Arrested at 1 cell zygotic stage	MEE 1	ND	Seq	0.3800	Endosperm development arrested
	MEE 2	ND	Seq	0.5600	Endosperm development arrested
	MEE 3	At2g21650*	Seq	0.0610	Endosperm development arrested
	MEE 4	At1g04630	Seq	0.0050	Endosperm development arrested
	MEE 5	At1g06220	Seq	0.4100	Endosperm development arrested
	MEE 6	At1g07890	Seq	0.0420	Endosperm development arrested
	MEE 7	At1g10470	Seq	0.4400	Endosperm development arrested
	MEE 8	At1g25310	Seq	0.1642	Endosperm development arrested
	MEE 9	At1g60870	Seq	0.3100	Endosperm development arrested
	MEE 10	At2g01200	Seq	0.5000	Endosperm development arrested
	MEE 11	At2g01620	Seq	0.0000	Endosperm development arrested
	MEE 12	At2g02955	Seq	0.4200	Endosperm development arrested
	MEE 13	At2g14680	Seq	0.0000	Endosperm development arrested
	MEE 14	At2g15890	Seq	0.0240	Endosperm development arrested
	MEE 15	At2g16970	Seq	0.4300	Endosperm development arrested
	MEE 16	At2g18650	Seq	0.0190	Endosperm normal
	MEE 17	At2g22250	Seq	0.3333	Endosperm normal
	MEE 18	At2g34090	Seq	0.2900	Endosperm development arrested
	MEE 19	At2g34130	Seq	0.4800	Endosperm development arrested
	MEE 20	At2g34220	Seq	0.1800	Endosperm development arrested
	MEE 21	At2g34570	Seq	0.3800	Endosperm development arrested
	MEE 22	At2g34780	Seq	0.4900	Endosperm development arrested
	MEE 23	At2g34790	Seq	0.3913	Endosperm development arrested
	MEE 24	At2g34830	Seq	0.2900	Endosperm development arrested
	MEE 25	At2g34850	Seq	0.3000	Endosperm development arrested
	MEE 26	At2g34870	Seq	0.4600	Endosperm development arrested
	MEE 27	At2g34880	Seq	0.4800	Endosperm development arrested
	MEE 28	At2g35210	Seq	0.4083	Endosperm development arrested
	MEE 29	At2g35340	Seq	0.4100	Endosperm development arrested
	MEE 30	At2g47470	Seq	0.2600	Endosperm development arrested
	MEE 31	At3g02570	Seq	0.5300	Endosperm development arrested
	MEE 32	At3g06350	Seq	0.5328	Endosperm development arrested
	MEE 33	At3g10920	Seq	0.0556	Endosperm normal
	MEE 34	At3g11270	Seq	0.2353	Endosperm normal
	MEE 35	At3g15030	Seq	0.0837	Endosperm development arrested
	MEE 36	At3g16440	Seq	0.0000	Endosperm development arrested
	MEE 37	At3g23440	Seq	0.3000	Endosperm development arrested
	MEE 38	At3g43160	Seq	0.4800	Endosperm development arrested
	MEE 39	At3g46330	Seq	0.4700	Endosperm development arrested
	MEE 40	At3g53700	Seq	0.4300	Endosperm development arrested
	MEE 41	At3g62670	Seq	0.1800	Endosperm development arrested
	MEE 42	At3g63080	Seq	0.5900	Endosperm development arrested
	MEE 43	At4g00020	Seq	0.5100	Endosperm normal
	MEE 44	At4g00060	Seq	0.2500	Endosperm development arrested
	MEE 45	At4g00260	Seq	0.3519	Endosperm development arrested
	MEE 46	At4g00310	Seq	0.2800	Endosperm development arrested
	MEE 47	At4g00950	Seq	0.3400	Endosperm development arrested
	MEE48	At4g14080*	ND	0.2200	Endosperm development arrested
	MEE 49	At4g01560	Seq	0.1900	Endosperm development arrested
	MEE 50	At4g00231	Seq	0.5700	Endosperm development arrested
	MEE 51	At4g04040	Seq	0.1000	Endosperm development arrested
	MEE 52	At4g04160	Seq	0.0435	Endosperm development arrested
	MEE 53	At4g10560	Seq	0.1100	Endosperm normal
	MEE 54	At4g11850	Seq	0.5500	Endosperm development arrested
	MEE 55	At4g13345	Seq	0.4600	Endosperm development arrested
	MEE 56	At4g13380	Seq	0.4300	Endosperm development arrested
	MEE 57	At4g13610	Seq	0.5800	Endosperm development arrested
	MEE 58	At4g13940	Seq	0.6593	Endosperm development arrested
	MEE 59	At4g37300	Seq	0.3093	Endosperm development arrested
	MEE 60	At5g05950	Seq	0.2500	Endosperm development arrested
	MEE 61	At5g14220	Seq	0.4000	Endosperm development arrested
	MEE 62	At5g45800	Seq	0.7714	Endosperm development arrested

Embryo development defects	MEE 63	At1g02140	Seq	0.8300	Embryos arrested at various stages of development
	MEE 64	At1g79860	Seq	0.4500	Arrested at 2-cell embryo stage
	MEE 65	At2g01280	Seq	0.6852	Arrested at early embryo stages
	MEE 66	At2g02240	Seq	0.2000	Arrested at 2-cell embryo stage
	MEE 67	At3g10110	Seq	0.3400	Arrested at 2-cell embryo stage
	MEE 68	At4g24660	Seq	0.0972	Arrested at early embryo stages
	MEE 69	At4g37140	Seq	0.2300	Arrested at 2-cell embryo stage
	MEE 70	At5g58230	Seq	0.0000	Abnormal embryo development

* Indicates sequences identified by TAIL-PCR but not yet confirmed by gene specific primers. ND, not determined