Transcriptional networks have been shown to play a primary role in regulating development in both plants and animals. It is through their exquisite control of gene expression in both space and time that pattern and form can be established and maintained. Research in the Brady lab focuses on understanding how a network of transcriptional interactions regulates tissue development and function. The root of the model plant, Arabidopsis thaliana, is ideally suited to study the architecture of transcriptional networks as most cell types are rotationally symmetrical and developmental time can be read along the root’s longitudinal axis, facilitating characterization of spatiotemporal development.
These features have been exploited using methods to characterize whole genome expression in individual cell types and developmental time. A rich set of transcriptional patterns were revealed to underly root spatiotemporal development, particularly in stele tissue. The stele is the central part of the root or stem and contains vascular and other associated tissue. Its primary function is to transport solutes and nutrients, and its mechanically strong secondary cell wall produced in some of its cell types is of great importance in papers, textiles and biofuel. Using this expression data coupled with high throughput technology, two types of interactions have been identified: 1) interactions between stele-enriched transcription factors and the promoters of their target transcription factors and 2) between transcription factors themselves (homo- or hetero-dimers). In the Brady lab, we continue to use this combination of systems biology, genomics and genetic approaches to determine how general and cell type-specific transcriptional regulators contribute to cell type and tissue specification, differentiation and function. We also explore the temporal dynamics of such regulatory modules.
Our research regarding the properties of tissue-enriched transcriptional networks can be applied to other organisms to determine the degree to which transcriptional networks that guide tissue development are conserved.
Allison Gaudinier received the UC Davis Dissertation Year Fellowship for 2016-2017.
Joel Rodríguez-Medina was awarded the UC MEXUS-CONACYT Doctoral Fellowship and has officially joined the Lab, March 2016
Concepcion Manzano received an Marie Skłodowska Curie Fellowship and has officially joined the Lab, Feb 2016
Allison Gaudinier was awarded the Eric and Louise Conn Biochemistry award, Feb 2016
Kaisa Kajala received the Chair's Postdoctoral Award, March 2015.
Don Gibson received UC Global Food Institute: Communication, Literacy, and Education for Agricultural Research (CLEAR) Traineeship, March 2015
Miguel de Lucas was awarded the 2015 Sabater by the Spanish Society of Plant Physiology
Kaisa Kajala won "Best Oral Presentation" award at ASPB Western Section, 2014.
Jasmine Garcha and Zachary Reynado received Outstanding Performance Citations by the College of Biological Sciences for their poster presentations, April 2014!
Michelle Tang received an NSF pre-doctoral fellowship, April 2014!
Bioinformatic Tools in Arabidopsis Research was published in Methods in Molecular Biology, 2014